A high-quality antibody demonstrates target specificity, selectivity, and sensitivity, enabling it to accurately detect the protein of interest even at low expression levels. Nevertheless, cross-reactivity with off-target proteins is still a concern with some antibodies. This challenge may present issues with experimental reproducibility, as non-specific antibodies can impede scientific progress.
MBLI introduces Knockout [KO] validation as the standard of antibody confirmation. The gene knockout validation is used to determine the function of a known sequence. By inactivating the gene that encodes the target protein, we can produce samples with a depletion of the target protein. KO validation is a highly effective technique that confirms the specificity of the antibody as depicted.
Production and Quality Control
A high-quality antibody demonstrates:
- Target specificity
- Target selectivity
- Target sensitivity
- Accurately detect the protein of interest even at low expression levels
Representative data demonstrating that the molecular weights measured with KO knockout validated Antibodies and wild-type antibodies:
Originally posted on: https://resources.mblintl.com/learning-center/research-area/knockout-antibodies
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