Anti-Human CD3-APC/CD4-FITC/CD8a-PerCP-Cyanine5.5[OKT-3,RPA-T4,OKT-8]

This product is a FCM antibody cocktail made up of APC Anti-Human CD3 Antibody [Clone: OKT-3] (Mouse IgG2a, κ), FITC Anti-Human CD4 Antibody [Clone: RPA-T4] (Mouse IgG1, κ) and PerCP/Cyanine5.5 Anti-Human CD8a Antibody [Clone: OKT-8] (Mouse IgG2a, κ).; CD3 is a heterotetrameric protein consisting of a CD3γ, a CDδ and 2 CD3ε. It forms complex with TCR. OKT-3 recognize human CD3ε. Human CD3 is expressed on the surface of T cells and NKT cells.; CD4 is also called Leu-3 or T4. It's a single-chain type I transmembrane glycoprotein, mainly expressed on the surface of T cells, and monocytes/macrophages. In T cells, CD4 forms complex with TCR/CD3 and play important roles in T cell immunity. The target of HIV is CD4+ T cells. Reduction of CD4+ T cells is the main reason of defected immunity after HIV infection.; CD8 is mainly expressed on cytotoxic T cells and also some subpopulations of NK cells. CD8 forms dimer function. In most cells, CD8 is a heterodimer consisting of CD8a and CD8b, but in NK cells nearly all CD8 is homodimer of CD8a. CD8a can form co-receptor with MHC-I restricted TCR to promote T cell antigen recognition and activation.

Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.

5 uL/Test

APC,FITC,PerCP/Cyanine5.5

For whole blood samples, add 5 uL Anti-Human CD3-APC/CD4-FITC/CD8a-PerCP-Cyanine5.5 Cocktail to 100 uL anticoagulant-treated blood sample. Mix and incubate the sample at 4°C in the dark for 30 min. Remove red blood cells with RBC lysis solution following the manufacturer's instruction. Wash the cell with cell staining buffer and discard the supernatant after centrifugation at 300 g for 5 min. Resuspend the cells with 200 uL cell staining buffer and load the sample on flow cytometer for detection. For other samples, 1x10^6 dissociated single cells are centrifuged at 300 g for 5 min with the supernatant discarded. Resuspend the cells with 100 uL cell staining buffer and add 5 uL Anti-Human CD3-APC/CD4-FITC/CD8a-PerCP-Cyanine5.5 Cocktail. Mix and incubate the sample at 4°C in the dark for 30 min. Add cell staining buffer to each tube, centrifuge at 300 g for 5 min and discard the supernatant. Resuspend the cells with 200 uL cell staining buffer and load the sample on flow cytometer for detection.