Hepatocyte Medium

Hepatocyte Medium (HM), when used with Hepatocyte Growth Supplement (HGS, Cat #5252) and 25 ml of fetal bovine serum (FBS) is a complete medium designed for optimal growth of normal hepatocytes in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (5%). The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the growth of normal hepatocytes in vitro.

HM consists of 500 ml of basal medium, 25 ml of fetal bovine serum (FBS, Cat. No. 0025), 5 ml of hepatocyte growth supplement (HGS, Cat. No. 5252) and 5 ml of Antibiotic Solution (P/S, Cat. No. 0503).